Open AccessConditional synthesis and utilization of 1,5-anhydroglucitol in Escherichia coli
Author(s) -
Yasuhiro Shiga,
Hideaki Mizuno,
Hiroshi Akanuma
Publication year - 1993
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.175.22.7138-7141.1993
Subject(s) - escherichia coli , biology , metabolism , polyol , biochemistry , enterobacteriaceae , biosynthesis , bacteria , carbohydrate metabolism , chemistry , gene , organic chemistry , genetics , polyurethane
A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retaining all the six carbon atoms, and only 5% was attributed to AG synthesized from C3 compounds. AG synthesis is apparent in an early stage of the stationary phase, and accumulation is transient both in cells and in medium. Evidence is also presented for AG uptake and metabolism and for effects of cyclic AMP.