Open AccessStructural organization of pBC1, a cryptic plasmid from Bacillus coagulans
Author(s) -
Edda De Rossi,
Anna Milano,
Patrizia Brigidi,
Francesca Bini,
Giovanna Riccardi
Publication year - 1992
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.174.2.638-642.1992
Subject(s) - replicon , bacillus coagulans , biology , plasmid , open reading frame , genetics , gene , nucleic acid sequence , reading frame , peptide sequence , origin of replication , bacteria
The complete nucleotide sequence of the Bacillus coagulans plasmid pBC1 was determined. The sequence revealed an open reading frame encoding a polypeptide of 259 amino acids. This open reading frame shows sequence similarity to genes coding for replication-associated proteins in a group of gram-positive bacterial plasmids known to replicate via single-stranded intermediates. A region required for replication in cis, when the intact replicon is supplied in trans, was identified as well.