Open AccessGenetic and morphological characterization of an Escherichia coli chromosome segregation mutant
Author(s) -
Philip S. Stewart,
Richard D’Ari
Publication year - 1992
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.174.13.4513-4516.1992
Subject(s) - nucleoid , biology , mutant , escherichia coli , genetics , mutation , allele , microbiology and biotechnology , chromosome , fluorescence microscope , chromosome segregation , fluorescence , gene , quantum mechanics , physics
The temperature-sensitive nucleoid segregation mutant of Escherichia coli, PAT32, formerly described as a parA mutant, has been shown to carry a mutation near 66 min on the genetic map. Fine mapping with phages from the collection of Kohara et al. is consistent with its being a parC allele. Observation by fluorescence microscopy revealed the formation, at a nonpermissive temperature, of filaments containing one or two large nucleoids and of normal-size anucleate cells. There was also a significant loss of viability.