Open AccessPhylogeny of the phototrophic rhizobium strain BTAi1 by polymerase chain reaction-based sequencing of a 16S rRNA gene segment
Author(s) -
Ji Young,
H. L. Downer,
Bertrand D. Eardly
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.7.2271-2277.1991
Subject(s) - biology , phototroph , phylogenetic tree , 16s ribosomal rna , bradyrhizobium japonicum , phylogenetics , proteobacteria , ribosomal rna , rhodopseudomonas , polymerase chain reaction , rhodopseudomonas palustris , genetics , botany , microbiology and biotechnology , bacteria , gene , symbiosis , rhizobiaceae
A 260-bp segment of the DNA that encodes 16S rRNA, corresponding to positions 44 to 337 in the Escherichia coli sequence, was amplified by the polymerase chain reaction and sequenced from each of 13 bacteria (rhizobia and purple phototrophs) in the alpha subdivision of the class Proteobacteria. The phylogenetic tree calculated from differences in the sequenced segment conforms well to our expectations based on other previously published data. The sequence from BTAi1 (a recently described phototrophic symbiont of the legume Aeschynomene) and that from the free-living phototroph Rhodopseudomonas palustris both fall within the range of variation found among strains of the soybean symbiont Bradyrhizobium japonicum. This suggests that it would be appropriate to include all of these organisms in a single genus.