Open AccessNucleotide and derived amino acid sequences of the major porin of Comamonas acidovorans and comparison of porin primary structures
Author(s) -
Susanne Gerbl-Rieger,
Jürgen Peters,
Josef Kellermann,
Friedrich Lottspeich,
Wolfgang Baumeister
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.7.2196-2205.1991
Subject(s) - porin , biology , bacterial outer membrane , peptide sequence , biochemistry , comamonas , amino acid , neurospora , nucleic acid sequence , protein primary structure , neurospora crassa , homology (biology) , genetics , gene , bacteria , escherichia coli , pseudomonas , mutant
The DNA sequence of the gene which codes for the major outer membrane porin (Omp32) of Comamonas acidovorans has been determined. The structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. Comparisons with amino acid sequences of outer membrane proteins and porins from several other members of the class Proteobacteria and of the Chlamydia trachomatis porin and the Neurospora crassa mitochondrial porin revealed a motif of eight regions of local homology. The results of this analysis are discussed with regard to common structural features of porins.