Open AccessHigh-frequency rearrangements in Rhizobium leguminosarum bv. phaseoli plasmids
Author(s) -
Susana Brom,
Alberto Santos,
M. L. Girard,
Guillermo Dávila,
Rafael Palacios,
David Romero
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.3.1344-1346.1991
Subject(s) - plasmid , biology , rhizobium leguminosarum , strain (injury) , rhizobium , genetics , genome , rhizobiaceae , microbiology and biotechnology , gene , bacteria , symbiosis , anatomy
High-frequency genomic rearrangements affecting the plasmids of Rhizobium leguminosarum bv. phaseoli CFN42 were analyzed. This strain contains six large plasmids ranging in size from 200 to 600 kb. In the absence of any selective pressure, we found 11 strains from 320 analyzed colonies that presented different kinds of plasmid-borne rearrangements, including sequence amplification, deletion, cointegration, and loss of plasmids. These data support the concept that the R. leguminosarum bv. phaseoli genome is a dynamic structure and imply that strains are mixtures of similar but not identical cells.