Open AccessPositive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius by use of 5-fluoroorotic acid
Author(s) -
Satoshi Kondo,
Akihiko Yamagishi,
Tairo Oshima
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.23.7698-7700.1991
Subject(s) - sulfolobus acidocaldarius , auxotrophy , biology , thermophile , uracil , mutant , biochemistry , microbiology and biotechnology , archaea , enzyme , gene , dna
Uracil auxotrophs of Sulfolobus acidocaldarius were positively selected by using 5-fluoroorotic acid. The wild-type strain was unable to grow in medium containing 5-fluoroorotic acid, whereas the mutants grew normally. Positive selection could be done for the auxotrophs. Mutants deficient in orotidine-5'-monophosphate pyrophosphorylase activity were isolated.