Open AccessTransport of nonmetabolizable opines by Agrobacterium tumefaciens
Author(s) -
Marimuthu Krishnan,
John W. Burgner,
William Scott Chilton,
Stanton B. Gelvin
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.2.903-905.1991
Subject(s) - octopine , agrobacterium tumefaciens , ti plasmid , biology , plasmid , agrobacterium , rhizobiaceae , genetics , bacteria , transformation (genetics) , gene , symbiosis
We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopaline) present in the bacterium. The transport of these opines required active cellular metabolism. Nonradioactive octopine, nopaline, and arginine competed effectively with [14C]octopine and [14C]nopaline for transport into A. tumefaciens A136, suggesting that the transport of these opines occurs via an arginine transport pathway not encoded by the Ti plasmid.