Open AccessSite-specific integration of the actinophage R4 genome into the chromosome of Streptomyces parvulus upon lysogenization
Author(s) -
Makoto Shirai,
Hiroki Nara,
Akio Satô,
Tokujiro Aida,
Hideo Takahashi
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.13.4237-4239.1991
Subject(s) - biology , genetics , genome , streptomyces coelicolor , chromosome , streptomyces , dna , gene , microbiology and biotechnology , bacteria
The lysogenization of Streptomyces parvulus by actinophage R4 occurs by site-specific integration of the phage genome into the chromosome. The DNA fragments containing the attachment sites on the host chromosome, the phage genome, and the two junctions created by insertion of the phage genome were cloned and sequenced. The attachment sites were found to share a common core of 12 bp. This common core sequence was not detected in chromosomal DNAs of S. coelicolor and S. lividans.