Open AccessIdentification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography
Author(s) -
B.L. Levinson,
KJ Kasyan,
SS Chiu,
T. C. Currier,
Jr Jm González
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.6.3172-3179.1990
Subject(s) - bacillus thuringiensis , exotoxin , biology , microbiology and biotechnology , pseudomonas exotoxin , serotype , colorado potato beetle , leptinotarsa , plasmid , bacillales , toxin , bacteria , pest analysis , botany , biochemistry , gene , genetics , pseudomonas aeruginosa , bacillus subtilis
An improved high-performance liquid chromatography separation was developed to detect and quantify beta-exotoxin production in Bacillus thuringiensis culture supernatants. Exotoxin production was assigned to a plasmid in five strains, from three subspecies (B. thuringiensis subsp. thuringiensis serotype 1, B. thuringiensis subsp. tolworthi serotype 9, and B. thuringiensis subsp. darmstadiensis serotype 10). A new exotoxin, called type II beta-exotoxin in this report, was discovered in B. thuringiensis subsp. morrisoni serotype 8ab, purified, and partially characterized. This material is more specific than type I beta-exotoxin and is very active against the Colorado potato beetle, Leptinotarsa decemlineata.