Open AccessCharacterization of the defect in the Escherichia coli mutT1 mutator gene
Author(s) -
S. K. Bhatnagar,
L C Bullions,
G. John Lew,
Maurice J. Bessman
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.5.2802-2803.1990
Subject(s) - biology , gene , genetics , escherichia coli , nucleic acid sequence , dna , microbiology and biotechnology , mutation
With a probe constructed from the wild-type gene, a DNA fragment containing the entire mutT1 mutator gene was isolated and cloned into pUC18. Nucleotide sequence analysis revealed that the mutator defect was most likely due to an IS1 insertion into the wild-type gene.