Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase | Zendy

Elizabeth Pradel | Zendy; Christian Marck | Zendy; P Boquet | Zendy

Open Access

Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase

Author(s) -

Elizabeth Pradel,

Christian Marck,

P Boquet

Publication year - 1990

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.172.2.802-807.1990

Subject(s) - biology , periplasmic space , nucleic acid sequence , peptide sequence , signal peptide , gene , microbiology and biotechnology , biochemistry , primer extension , consensus sequence , escherichia coli , nucleotide

The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a calculated Mr of 43,514. Its promoter was defined by primer extension of the mRNA made in vivo. Like many genes under positive control, its -35 promoter region does not match the consensus. The agp gene is both preceded and followed by transcription termination signals, so it appears to be transcribed as a single unit.

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