Open AccessDNA sequences required for the alkalophily of Bacillus sp. strain C-125 are located close together on its chromosomal DNA
Author(s) -
Toshiaki Kudo,
Motohiro Hino,
Masashi Kitada,
Koki Horikoshi
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.12.7282-7283.1990
Subject(s) - biology , strain (injury) , dna , plasmid , mutant , microbiology and biotechnology , circular bacterial chromosome , genetics , gene , dna replication , anatomy
Two alkali-sensitive mutants of alkalophilic Bacillus sp. strain C-125 were obtained. Mutant 38154 showed defective regulation of internal pH. Plasmids pALK1 and pALK2, containing DNA fragments different from those of the parent strain, were able to recover alkalophily in mutants 18224 and 38154, respectively. DNA analysis suggested that the two fragments overlapped on the chromosomal DNA of strain C-125.