Open AccessIsolation and characterization of lipoteichoic acid, a cell envelope component involved in preventing phage adsorption, from Lactococcus lactis subsp. cremoris SK110
Author(s) -
L. Sijtsma,
Jan Wouters,
K. J. Hellingwerf
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.12.7126-7130.1990
Subject(s) - lactococcus lactis , lipoteichoic acid , cell envelope , biology , teichoic acid , galactose , biochemistry , cell wall , bacteria , microbiology and biotechnology , chromatography , lactic acid , chemistry , escherichia coli , peptidoglycan , staphylococcus aureus , genetics , gene
The cell envelope of the phage-resistant Lactococcus lactis subsp. cremoris SK110 differed from its phage-sensitive variant by the presence of a galactosyl-containing component. This component was present in material obtained from SK110 by a mild alkali treatment. In a similar fraction extracted from SK112, no galactosyl-containing components were detected. With respect to gel permeation chromatography and electrophoretic mobility, identical characteristics of the alkali-extracted material and purified lipoteichoic acid (LTA) were measured. Chemical analysis of the latter component showed the absence of galactose in LTA isolated from SK112, whereas it was present in LTA obtained from SK110. In this paper, we propose that galactosyl-containing LTA is involved in preventing phage adsorption to L. lactis subsp. cremoris SK110.