Open AccessRecombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases
Author(s) -
Sean D. Colloms,
Peter Sýkora,
George Szatmari,
David J. Sherratt
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.12.6973-6980.1990
Subject(s) - biology , recombinase , site specific recombination , cole1 , genetics , integrase , gene , holliday junction , plasmid , integrases , escherichia coli , nucleic acid sequence , bacteriophage , gene conversion , recombination , microbiology and biotechnology
Site-specific recombination at the plasmid ColE1 cer site requires the Escherichia coli chromosomal gene xerC. The xerC gene has been localized to the 85-min region of the E. coli chromosome, between cya and uvrD. The nucleotide sequences of the xerC gene and flanking regions have been determined. The xerC gene encodes a protein with a calculated molecular mass of 33.8 kDa. This protein has substantial sequence similarity to the lambda integrase family of site-specific recombinases and is probably the cer recombinase. The xerC gene is expressed as part of a multicistronic unit that includes the dapF gene and two other open reading frames.