Open AccessThe gdhA gene is located at 38.6 minutes on the Escherichia coli map
Author(s) -
Su Young Kim,
Debbie McLaggan,
Wolfgang Epstein
Publication year - 1990
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.172.10.6127-6128.1990
Subject(s) - biology , escherichia coli , complementation , genetics , gene , microbiology and biotechnology , mutant
We report here that the gdhA gene of Escherichia coli, which encodes the NADP-specific glutamate dehydrogenase, is located at 38.6 min on the map. We have confirmed this location by showing linkage with three Tn10 insertions that are linked to the aroD, pheS, and ansA loci, by complementation by a restriction-mapped lambda clone, and by showing correspondence between the restriction maps of the chromosome and the cloned and sequenced gdhA gene.