Open AccessOverproduced bacteriophage T4 gene 33 protein binds RNA polymerase
Author(s) -
Kelly P. Williams,
Rolf Müller,
Wolfgang Rüger,
E. Peter Geiduschek
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.6.3579-3582.1989
Subject(s) - biology , rna polymerase , microbiology and biotechnology , sigma factor , polymerase , bacteriophage , transcription (linguistics) , rna polymerase ii , gene , escherichia coli , rna dependent rna polymerase , virology , gene expression , genetics , promoter , linguistics , philosophy
Bacteriophage T4 gene 33 protein (gp33), which is required for viral late transcription, has been overproduced. The purified gp33 binds to RNA polymerase core from uninfected or T4-infected Escherichia coli, but the major E. coli transcription initiation factor, sigma 70, competed effectively for this binding.