Open AccessCloning and sequencing of the gene encoding cytochrome c553 from Desulfovibrio vulgaris Hildenborough
Author(s) -
Gijs J. H. van Rooijen,
Mireille Bruschi,
Gerrit Voordouw
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.6.3575-3578.1989
Subject(s) - desulfovibrio vulgaris , biology , gene , nucleic acid sequence , genetics , peptide sequence , dna , cytochrome , microbiology and biotechnology , desulfovibrio , biochemistry , bacteria , enzyme
The gene encoding cytochrome c553 from Desulfovibrio vulgaris Hildenborough was cloned by using two synthetic deoxyoligonucleotide probes. The amino acid sequence derived from the sequence of the gene differs from that reported by Bruschi and LeGall (Biochim. Biophys. Acta 271:48-60, 1972). Renewed protein sequencing confirmed the correctness of the DNA-derived sequence. The gene sequence indicates cytochrome c553 to be synthesized as a precursor protein with an NH2-terminal signal sequence of 24 residues.