Open AccessStreptomyces lipmanii expresses two restriction systems that inhibit plasmid transformation and bacteriophage plaque formation
Author(s) -
Patti Matsushima,
Richard H. Baltz
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.6.3128-3132.1989
Subject(s) - biology , plasmid , bacteriophage , transformation (genetics) , streptomyces , streptomycetaceae , mutant , strain (injury) , microbiology and biotechnology , restriction enzyme , restriction fragment , dna , restriction map , actinomycetales , genetics , bacteria , gene , escherichia coli , anatomy
Bacteriophage host range studies suggested that several beta-lactam-producing streptomycetes express similar restriction-modification systems. Streptomyces lipmanii LE32 expressed two restriction-modification systems, designated SliI and SliII. A mutant strain, PM87, was defective only in SliI restriction but expressed both SliI and SliII modification. Streptomyces sp. strain A57986, a natural isolate partially deficient in the expression of SliI and SliII restriction, nevertheless modified bacteriophage DNA for both SliI and SliII specificities. Protoplasts of PM87 and A57986 were transformed by several plasmids, and the modified plasmids isolated from these strains transformed wild-type S. lipmanii efficiently.