Open AccessIdentification and sequence of the basic replication region of a broad-host-range plasmid isolated from Thiobacillus ferrooxidans
Author(s) -
Rosemary A. Dorrington,
Douglas E. Rawlings
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.5.2735-2739.1989
Subject(s) - biology , plasmid , genetics , origin of replication , homology (biology) , autonomously replicating sequence , escherichia coli , nucleic acid sequence , dna , base pair , microbiology and biotechnology , gene
The minimum region required for replication of the broad-host-range Thiobacillus ferrooxidans plasmid pTF-FC2 in Escherichia coli was shown to be contained on a 2.05-kilobase fragment of DNA. A 184-base-pair fragment that was required in cis for plasmid replication was identified. This region was also involved in plasmid incompatibility. Nucleotide sequencing of this region revealed three perfectly conserved 22-base-pair tandemly repeated sequences. A comparison of this region with the equivalent region of the broad-host-range plasmid R1162 showed that the repeated sequences had 60% nucleotide homology. The 106-base-pair region immediately adjacent to the repeated sequences was 75% homologous. These plasmids were compatible.