Open AccessParticipation of Escherichia coli heat shock proteins DnaJ, DnaK, and GrpE in P1 plasmid replication
Author(s) -
Kit Tilly,
Michael B. Yarmolinsky
Publication year - 1989
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.171.11.6025-6029.1989
Subject(s) - plasmid , biology , escherichia coli , prophage , transformation (genetics) , genetics , heat shock protein , dna replication , origin of replication , low copy number , heat shock , gene , microbiology and biotechnology , bacteriophage
Low-copy-number plasmids, such as P1 prophage and the fertility factor F, require a plasmid-encoded replication protein and several host products for replication. Stable maintenance also depends on active partitioning of plasmids into daughter cells. Mini-P1 par+ and par plasmids were found to be destabilized by mutations in the dnaJ, dnaK, and grpE genes of Escherichia coli. The transformation efficiency and stability of mini-F plasmids were also reduced in the mutant strains. These results indicate that heat shock proteins DnaJ, DnaK, and GrpE play roles in the replication of plasmid P1 and probably also in of F.