Open AccessCloning of DNA involved in sporulation of Streptomyces griseus
Author(s) -
Martin J. Babcock,
Kathleen E. Kendrick
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.6.2802-2808.1988
Subject(s) - biology , streptomyces griseus , streptomyces coelicolor , mutant , genetics , dna , cloning (programming) , molecular cloning , restriction map , microbiology and biotechnology , plasmid , cloning vector , streptomyces , gene , bacteria , peptide sequence , computer science , programming language
Twenty-two bald mutants of Streptomyces griseus were isolated and classified into four phenotypic groups, two of which showed conditional sporulation. A 3-kilobase fragment of DNA was cloned in a high-copy-number vector and detected by its ability to restore sporulation to one class of conditionally bald mutants. Analysis of subclones demonstrated that the sporulation property was contained within a 2.5-kilobase fragment. Hybridization studies and restriction analysis indicated that this DNA fragment was present in several Streptomyces species and was distinct from DNA that has been shown to complement afsA mutants of S. bikiniensis and bldA mutants of S. coelicolor.