Open AccessA clinical isolate of transposon Tn5 expressing streptomycin resistance in Escherichia coli
Author(s) -
Olga Genilloud,
Jesús Blázquez,
Philippe Mazodier,
Felipe Moreno
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.3.1275-1278.1988
Subject(s) - biology , transposable element , streptomycin , escherichia coli , genetics , dna transposable elements , microbiology and biotechnology , transposon mutagenesis , gene , mutation , antibiotics , mutant
The central region of transposon Tn5 carries three antibiotic resistance markers: neo, ble, and str. The str gene codes for a phosphotransferase that inactivates streptomycin. This activity is phenotypically expressed in several gram-negative bacteria but not in Escherichia coli. We identified a Tn5 variant in E. coli clinical isolates that express streptomycin resistance. This transposon carries a 6-base-pair deletion within the str gene, near the 3' end. The same kind of mutation had been previously obtained experimentally from Tn5.