Open AccessIsolation of additional monoclonal antibodies directed against cell surface antigens of Myxococcus xanthus cells undergoing submerged development
Author(s) -
James S. Gill,
Martin Dworkin
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.12.5953-5955.1988
Subject(s) - myxococcus xanthus , monoclonal antibody , biology , antigen , antibody , pan t antigens , microbiology and biotechnology , lipopolysaccharide , cell , blot , immunology , biochemistry , gene , mutant
Thirteen additional monoclonal antibodies directed against cell surface antigens of Myxococcus xanthus cells undergoing submerged development were isolated and partially characterized. As measured by quantitative enzyme-linked immunosorbent assay, 10 of these antibodies recognized antigens common to both vegetatively growing cells and cells undergoing submerged development; 3 antibodies recognized antigens specific to developing cells. Five antigens were revealed as single bands on Western blots (immunoblots), and one produced multiple, diffuse bands characteristic of lipopolysaccharide.