Open AccessFactors affecting transfer frequency of pAM beta 1 from Streptococcus faecalis to Lactobacillus plantarum
Author(s) -
Yasuko Sasaki,
Naoki Taketomo,
Takashi Sasaki
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.12.5939-5942.1988
Subject(s) - lactobacillus plantarum , biology , membrane filter , microbiology and biotechnology , enterococcus faecalis , lactobacillus , streptococcus , membrane , bacteria , escherichia coli , biochemistry , lactic acid , genetics , gene
Conjugal transfer of streptococcal plasmid pAM beta 1 to Lactobacillus plantarum by a filter mating method was examined under various conditions. The transfer frequency depended greatly on the type, pore size, and side (front or back) of the membrane filter. The passage of sterilized water through the membrane under reduced pressure after donor and recipient cells were trapped on it increased the transfer frequency about 10-fold.