Open AccessSynthesis of bacilysin by Bacillus subtilis branches from prephenate of the aromatic amino acid pathway
Author(s) -
M D Hilton,
N. Gürdal Alaeddinoğlu,
Arnold L. Demain
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.1.482-484.1988
Subject(s) - bacillus subtilis , biology , mutant , biosynthesis , biochemistry , aromatic amino acids , microbiology and biotechnology , amino acid , bacteria , enzyme , gene , genetics
Previously characterized mutants of Bacillus subtilis 168 were analyzed for the accumulation of the antibiotic bacilysin in culture broths and for the presence of bacilysin synthetase in cell extracts. All aro mutants tested were deficient in bacilysin biosynthesis but had synthetase activity. Mutants with lesions in tyrA and pheA produced normal levels of bacilysin, suggesting that prephenate is the primary metabolic precursor of bacilysin.