Open AccessCloning and sequencing of a gene encoding an aminoglycoside 6'-N-acetyltransferase from an R factor of Citrobacter diversus
Author(s) -
Fred C. Tenover,
David Filpula,
Kenneth L. Phillips,
James J. Plorde
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.1.471-473.1988
Subject(s) - biology , sisomicin , plasmid , pbr322 , genetics , gene , nucleic acid sequence , citrobacter , acetyltransferase , cloning (programming) , open reading frame , chloramphenicol acetyltransferase , escherichia coli , microbiology and biotechnology , peptide sequence , enterobacteriaceae , tobramycin , gene expression , reporter gene , bacteria , acetylation , computer science , pseudomonas aeruginosa , programming language
The aacA1 gene, which encodes a 6'-N-acetyltransferase [AAC(6')-I] mediating resistance to kanamycin, tobramycin, and amikacin, was cloned from the Citrobacter diversus R plasmid pBWH100 into the Escherichia coli vector pBR322. The complete nucleotide sequence of the gene and flanking regions was determined. A protein of approximately 21 kilodaltons was identified when the chimeric plasmid encoding the aacA1 gene was introduced into E. coli maxicells. This value is consistent with the size predicted for a protein translated from the open reading frame of the gene.