Open AccessIsolation and sequencing of a genomic clone encoding aspartic proteinase of Rhizopus niveus
Author(s) -
Hiroyuki Horiuchi,
Koji Yanai,
Taro Okazaki,
Masamichi Takagi,
Keiji Yano
Publication year - 1988
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.170.1.272-278.1988
Subject(s) - biology , peptide sequence , nucleic acid sequence , amino acid , gene , genetics , biochemistry , sequence alignment , intron , aspartic acid , coding region , microbiology and biotechnology
A gene encoding Rhizopus niveus aspartic proteinase was isolated from an R. niveus genomic library by using oligonucleotides probes corresponding to its partial amino acid sequence, and its nucleotide sequence was determined. By comparing its deduced amino acid sequence with the amino acid sequence of rhizopuspepsin (5, 26), we concluded that the R. niveus aspartic proteinase gene has an intron within its coding region and that it has a preproenzyme sequence of 66 amino acids upstream of the mature enzyme of 323 amino acids.