Open AccessIsolation and nucleotide sequence of the methanol dehydrogenase structural gene from Paracoccus denitrificans
Author(s) -
N. Harms,
G.E de Vries,
KarlHeinz Maurer,
Jasper Hoogendijk,
A. H. Stouthamer
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.9.3969-3975.1987
Subject(s) - paracoccus denitrificans , methanol dehydrogenase , biology , nucleic acid sequence , microbiology and biotechnology , gene , structural gene , clone (java method) , genomic dna , biochemistry , enzyme , escherichia coli
A genomic clone bank of Paracoccus denitrificans DNA has been constructed in the expression vector set pEX1, pEX2, and pEX3. Screening of this clone bank with antibodies raised against P. denitrificans methanol dehydrogenase resulted in the isolation of a clone, pNH3, that synthesized methanol dehydrogenase cross-reactive proteins. The nucleotide sequence of the P. denitrificans DNA fragment inserted in this clone has been determined and shown to contain the full methanol dehydrogenase structural gene. DNA cross-hybridization was found with DNA fragments which have been reported to contain the methanol dehydrogenase structural genes from Methylobacterium sp. strain AM1 and Methylobacterium organophilum.