Open AccessDecreased chromate uptake in Pseudomonas fluorescens carrying a chromate resistance plasmid
Author(s) -
Hisao Ohtake,
Carlos Cervantes,
Simón Silver
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.8.3853-3856.1987
Subject(s) - pseudomonas fluorescens , strain (injury) , biology , chromate conversion coating , plasmid , pseudomonas , pseudomonadales , pseudomonadaceae , microbiology and biotechnology , kinetics , cysteine , biochemistry , bacteria , enzyme , chromium , chemistry , dna , anatomy , genetics , physics , organic chemistry , quantum mechanics
CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more resistant to CrO4(2-) than those grown with 0.15 mM djenkolic acid, which derepressed the 35SrO4(2-) uptake system. Kinetics of 51CrO4(2-) uptake by P. fluorescens with and without the plasmid showed that the Vmax for 51CrO4(2-) uptake with the resistant strain was 2.2 times less than the Vmax for the sensitive strain, whereas the Km remained constant.