Open AccessLocations of the hydrogenases of Methanobacterium formicicum after subcellular fractionation of cell extract
Author(s) -
Stephen F. Baron,
David P. Brown,
James G. Ferry
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.8.3823-3825.1987
Subject(s) - hydrogenase , biology , methanobacterium , fractionation , membrane , biochemistry , cell fractionation , sepharose , chromatography , enzyme , chemistry , archaea , gene
The F420 hydrogenase of Methanobacterium formicicum was associated with membranes isolated by sucrose density gradient ultracentrifugation of cell extract. The methyl viologen hydrogenase was present in the soluble fractions. Column chromatography with phenyl-Sepharose CL-4B revealed that the F420 hydrogenase was strongly hydrophobic, suggesting that it associates with isolated membranes through hydrophobic interactions.