Open AccessTn5 insertion mutants of Pseudomonas aeruginosa deficient in surface expression of ferripyochelin-binding protein
Author(s) -
Pamela A. Sokol
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.7.3365-3368.1987
Subject(s) - biology , mutant , pseudomonas aeruginosa , transposable element , microbiology and biotechnology , pseudomonadaceae , pseudomonadales , binding protein , monoclonal antibody , pseudomonas , bacteria , biochemistry , antibody , gene , genetics
Transposon (Tn5) insertion mutants were isolated in Pseudomonas aeruginosa PAO. These mutants were screened for expression of the ferripyochelin-binding protein with monoclonal antibody in a whole-cell immunoblot assay. Fourteen mutants were identified which did not express ferripyochelin-binding protein on the cell surface. These mutants did not take up 59Fe-labeled pyochelin and grew slowly in the presence of iron chelators.