Open AccessCharacterization of Vibrio fischeri rRNA operons and subcloning of a ribosomal DNA promoter
Author(s) -
Dorit Amikam,
Jonathan Kuhn
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.5.2318-2321.1987
Subject(s) - biology , subcloning , ribosomal rna , 23s ribosomal rna , operon , microbiology and biotechnology , 5s ribosomal rna , plasmid , ribosomal dna , genetics , 18s ribosomal rna , gene , rna , escherichia coli , ribosome , phylogenetics
Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical with regard to cleavage sites for various restriction endonucleases. A DNA library was constructed, and three ribosomal DNA clones were obtained. One of these clones contained an entire rRNA operon and was used as a source for subcloning. The promoter region which leads to plasmid instability was successfully subcloned into pHG165. The terminator region was subcloned into pBR322.