Open AccessPromoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase
Author(s) -
Tyrrell Conway,
Yehia A. Osman,
J. I. Konnan,
E. Hoffmann,
L. O. Ingram
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.3.949-954.1987
Subject(s) - zymomonas mobilis , pyruvate decarboxylase , biology , escherichia coli , biochemistry , promoter , nucleic acid sequence , peptide sequence , gene , carboxy lyases , amino acid , dna , microbiology and biotechnology , enzyme , gene expression , ethanol fuel , fermentation , alcohol dehydrogenase
DNA sequence analysis showed that pyruvate decarboxylase (one of the most abundant proteins in Zymomonas mobilis) contains 559 amino acids. The promoter for the gene encoding pyruvate decarboxylase was not recognized by Escherichia coli, although the cloned gene was expressed at relatively high levels under the control of alternative promoters. The promoter region did not contain sequences which could be identified as being homologous to the generalized promoter structure for E. coli. Hydropathy plots for the amino acid sequence indicated that pyruvate decarboxylase contains a large number of hydrophobic domains which may contribute to the thermal stability of this enzyme.