Open AccessRequirement of a heat-labile factor(s) for in vitro expression of the amp gene of pBR322
Author(s) -
Yoshitaka Kuriki
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.12.5856-5858.1987
Subject(s) - biology , pbr322 , escherichia coli , gene , in vitro , gene expression , transcription (linguistics) , microbiology and biotechnology , plasmid , biochemistry , linguistics , philosophy
The amp gene of pBR328 and pBR322 was expressed less efficiently at 45 than at 30 degrees C in an in vitro coupled transcription-translation system of Escherichia coli. Preincubation of S30 extract at 45 degrees C reduced specifically its ability to express the amp gene at 30 degrees C, indicating inactivation of a factor(s) required for efficient expression of the amp gene.