Open AccessCloning of whiG, a gene critical for sporulation of Streptomyces coelicolor A3(2)
Author(s) -
Cármen Méndez,
Keith F. Chater
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.12.5715-5720.1987
Subject(s) - streptomyces coelicolor , biology , subcloning , plasmid , temperateness , streptomyces , genetics , cloning (programming) , gene , mutant , microbiology and biotechnology , bacteriophage , escherichia coli , bacteria , computer science , programming language
In whiG mutants of Streptomyces coelicolor A3(2), aerial hyphae do not show any sign of sporulation. A library of S. coelicolor DNA was prepared in a phi C31 temperate phage vector (KC516), and one recombinant phage (KC750) that could restore the wild-type phenotype to a collection of whiG mutants when integrated into their genomes was found. Subcloning experiments with low- and high-copy-number Streptomyces plasmid vectors allowed partial localization of whiG in the cloned DNA and revealed that hypersporulation was associated with the presence of extra copies of whiG.