Open AccessMapping of the constitutive lysyl-tRNA synthetase gene of Escherichia coli K-12
Author(s) -
Ralf Emmerich,
Irvin N. Hirshfield
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.11.5311-5313.1987
Subject(s) - biology , escherichia coli , isoelectric point , gene , microbiology and biotechnology , transfer rna , mutant , gel electrophoresis , genetics , structural gene , biochemistry , rna , enzyme
The constitutive lysyl-tRNA synthetase gene (lysS) was mapped at 62.1 min on the Escherichia coli chromosome by a combination of conjugation and transduction, with physical confirmation by two-dimensional gel electrophoresis. Revertant analysis suggests that the altered isoelectric point and the low amount of the mutant LysS protein may be due to a single mutational event.