Open AccessChromosomal location of TOL plasmid DNA in Pseudomonas putida
Author(s) -
Martha Irvine Sinclair,
Paulhallettandanthony Maxwell,
B. R. Lyon,
Bruce Holloway
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.168.3.1302-1308.1986
Subject(s) - biology , pseudomonas putida , plasmid , pseudomonas , bacterial conjugation , chromosome , genetics , dna , gene , circular bacterial chromosome , pseudomonadaceae , microbiology and biotechnology , bacteria , escherichia coli
The soil isolate Pseudomonas putida MW1000 can grow on toluene and other hydrocarbons; in this respect it is similar to strains of Pseudomonas which carry the TOL plasmid. By conjugation experiments, the genes conferring these growth abilities have been shown to be located on the bacterial chromosome, linked to vil and catB. A 56-kilobase segment of the bacterial chromosome of MW strains carrying the TOL genes can transpose to the IncP-1 plasmid R18-18. Physical analysis of these TOL R18-18 hybrids has shown that the TOL segment is almost identical to the same region found in the TOL plasmid pWW0.