Open AccessNew role for photoreversible pyrimidine dimers in induction of prototrophic mutations in excision-deficient Escherichia coli by UV light
Author(s) -
Manuel Ruiz-Rubio,
Roger Woodgate,
B.A. Bridges,
Guadalupe Herrera,
Manuel Blanco
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.166.3.1141-1143.1986
Subject(s) - pyrimidine dimer , biology , repressor lexa , escherichia coli , repressor , auxotrophy , mutagenesis , bacteria , pyrimidine , mutation , biochemistry , microbiology and biotechnology , genetics , gene , dna repair , gene expression
UV mutagenesis to His+ in certain recA441 lexA51 bacteria was not photoreversible, indicating that pyrimidine dimers are not target lesions. Photoreversibility was observed in recA+ lexA51 bacteria, showing that pyrimidine dimers are needed to activate the recA+ protein (unlike the recA441 protein) to perform a function in UV mutagenesis distinct from cleavage of the lexA repressor.