Open AccessCloning of a gene encoding choline transport in Saccharomyces cerevisiae
Author(s) -
Jùnichi Nikawa,
Y. Tsukagoshi,
Shinji Yamashita
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.166.1.328-330.1986
Subject(s) - biology , complementation , plasmid , saccharomyces cerevisiae , gene , yeast , mutant , genetics , molecular cloning , cloning (programming) , restriction map , dna , genomic library , microbiology and biotechnology , peptide sequence , computer science , programming language
By genetic complementation in a yeast choline transport mutant from a yeast gene library, we isolated plasmids encoding choline transport. The cloned plasmids contained a common 4.0-kilobase DNA fragment and also complemented an ethanolamine transport defect. The cloned sequence present in the yeast genome was possibly unique.