Open AccessConservation of DNA sequences for plasmid-mediated citrate utilization within the enterobacteria
Author(s) -
T Hirato,
Naotaka Ishiguro,
Morikazu Shinagawa,
Gihei Sato
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.165.1.324-327.1986
Subject(s) - biology , plasmid , citrobacter freundii , enterobacter aerogenes , southern blot , microbiology and biotechnology , dna , klebsiella pneumoniae , plasmid preparation , enterobacteriaceae , restriction map , salmonella enteritidis , hybrid plasmid , salmonella , homologous chromosome , pbr322 , gene , genetics , escherichia coli , bacteria
Southern blot DNA-DNA hybridization experiments with a cloned Cit+ DNA fragment as a probe showed that the plasmid-mediated Cit+ determinants from four Cit plasmids (R726, pOH3001, pOH3035, and pOH30221) were all homologous. Sequences homologous to the plasmid-borne Cit+ gene were also found in total bacterial DNA isolated from Salmonella paratyphi B, Salmonella enteritidis, Salmonella typhimurium LT-2, Citrobacter freundii, ATCC 8090, Citrobacter amalonaticus ATCC 25405, Klebsiella pneumoniae I and IID 977, and Enterobacter aerogenes ATCC 13048. The DNA digest from C. amalonaticus ATCC 25405 contained a 1.4-kilobase BamHI-HincII DNA fragment that was strongly homologous with and identical in size to the plasmid Cit+ probe.