Open AccessPosttranscriptional autoregulation of Escherichia coli threonyl tRNA synthetase expression in vivo
Author(s) -
J. Scott Butler,
Mathias Springer,
J. Dondon,
Marianne GrunbergManago
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.165.1.198-203.1986
Subject(s) - biology , mutant , transfer rna , escherichia coli , microbiology and biotechnology , overproduction , in vivo , gene , mutation , gene expression , genetics , rna
Five mutations in thrS, the gene for threonyl-tRNA synthetase, have been characterized, and the sites of the mutations have been localized to different regions of the thrS gene by recombination with M13 phage carrying portions of the thrS gene. Quantitative immunoblotting shows that some of these mutations cause the overproduction of structurally altered threonyl-tRNA synthetase in vivo. The amounts of in vivo thrS mRNA as measured by quantitative hybridization are, however, the same as wild-type levels for each mutant. These results demonstrate that the expression of threonyl-tRNA synthetase is autoregulated at the posttranscriptional level in vivo.