Molecular cloning and expression of the xylanase gene of alkalophilic Bacillus sp. strain C-125 in Escherichia coli | Zendy

Hiroshi Honda | Zendy; Toshiaki Kudo | Zendy; Koki Horikoshi | Zendy

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Molecular cloning and expression of the xylanase gene of alkalophilic Bacillus sp. strain C-125 in Escherichia coli

Author(s) -

Hiroshi Honda,

Toshiaki Kudo,

Koki Horikoshi

Publication year - 1985

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.161.2.784-785.1985

Subject(s) - pbr322 , biology , xylanase , escherichia coli , plasmid , strain (injury) , molecular cloning , bacillaceae , microbiology and biotechnology , hindiii , enterobacteriaceae , gene , cloning (programming) , bacillus (shape) , recombinant dna , bacteria , biochemistry , genetics , gene expression , enzyme , bacillus subtilis , anatomy , computer science , programming language

The gene for xylanase A of alkalophilic Bacillus sp. strain C-125 was cloned in Escherichia coli with pBR322. The plasmid pCX311 contained 2.6- and 2.0-kilobase-pair HindIII fragments. The characteristics of the purified pCX311-encoded xylanase were the same as those of purified xylanase A from alkalophilic Bacillus sp. strain C-125.

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