Plasmid-borne sulfonamide resistance determinants studied by restriction enzyme analysis | Zendy

Göte Swedberg | Zendy; Ola Sköld | Zendy

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Plasmid-borne sulfonamide resistance determinants studied by restriction enzyme analysis

Author(s) -

Göte Swedberg,

Ola Sköld

Publication year - 1983

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.153.3.1228-1237.1983

Subject(s) - plasmid , biology , dihydropteroate synthase , sulfonamide , gene , microbiology and biotechnology , restriction enzyme , genetics , drug resistance , bacteria , enzyme , restriction fragment length polymorphism , polymerase chain reaction , biochemistry , chemistry , pyrimethamine , malaria , stereochemistry , immunology , plasmodium falciparum

The relationship between sulfonamide resistance genes carried on different plasmids was investigated by restriction enzyme analysis and DNA-DNA hybridization. The results showed that sulfonamide resistance mediated by different plasmids is determined by the production of at least two different types of drug-resistant dihydropteroate synthase. Plasmids pGS01, pGS02, and R22259, found in bacteria isolated from patients in Swedish hospitals, contained identical sulfonamide resistance genes, which were also identical to those of plasmids R1, R100, R6, and R388. These latter plasmids, which have been well studied in different laboratories, were originally from clinical isolates from different parts of the world. Two other clinically isolated plasmids, pGS04 and pGS05, were shown to contain sulfonamide resistance determinants of a completely different type.

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