Molecular cloning of the region of the terminus of Escherichia coli K-12 DNA replication | Zendy

Samír Béjar | Zendy; JeanPierre Bouché | Zendy

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Molecular cloning of the region of the terminus of Escherichia coli K-12 DNA replication

Author(s) -

Samír Béjar,

JeanPierre Bouché

Publication year - 1983

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.153.2.604-609.1983

Subject(s) - biology , cosmid , plasmid , escherichia coli , southern blot , molecular cloning , restriction enzyme , restriction map , microbiology and biotechnology , genetics , dna , gene , complementary dna

A series of plasmids have been isolated either by ligation of defined restriction fragments to plasmid pBR325 or by screening of a cosmid bank by in situ colony hybridization. Together with one previously isolated plasmid, they spanned 86% of the 30.5- to 34-min region of the genetic map of Escherichia coli K-12. Physical analysis of these plasmids and hybridizations to Southern blots confirmed the endonuclease map of this region, with the exception of a 9.3-kilobase pair inversion.

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