Open AccessIdentification of two genes immediately downstream from the polA gene of Escherichia coli
Author(s) -
Catherine M. Joyce,
Nigel D. F. Grindley
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.152.3.1211-1219.1982
Subject(s) - biology , gene , escherichia coli , genetics , plasmid , transcription (linguistics) , coding region , microbiology and biotechnology , base pair , nucleic acid sequence , dna , genome , philosophy , linguistics
We have identified two genes within a 1-kilobase region immediately following the polA gene of Escherichia coli. The first, whose transcription is initiated about 150 base pairs beyond the end of the polA coding sequence, is the gene corresponding to the previously sequenced "spot 42 RNA" (B. G. Sahagan and J. E. Dahlberg, J. Mol. Biol. 131:573--592, 1979). The second, located further downstream and transcribed towards polA, is the structural gene for a 22-kilodalton polypeptide, which we have detected by using plasmid-directed protein synthesis in maxicells. Sequence analysis of this region of the E. coli genome suggests that it contains little, if any, redundant DNA.