Open AccessCloning of the trp-1 gene from neurospora crassa by complementation of a trpC mutation in Escherichia coli
Author(s) -
J K Keesey,
John A. DeMoss
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.152.2.954-958.1982
Subject(s) - biology , neurospora crassa , complementation , plasmid , mutant , microbiology and biotechnology , escherichia coli , hybrid plasmid , pbr322 , genetics , dna , biochemistry , gene
Studies with a hybrid plasmid containing 4.0 kilobase pairs of Neurospora crassa DNA cloned into plasmid pBR322 indicated that the plasmid restored to prototrophy a trpC mutant of Escherichia coli which lacked phosphoribosyl anthranilate isomerase but not a trpC mutant which lacked indole glycerol phosphate synthetase, that the relevant transcription was initiated at a promoter within the N. crassa DNA, and that the phosphoribosyl anthranilate isomerase could be specified by a subcloned segment of the original DNA.