Open AccessAn amber mutation in the gene encoding the beta' subunit of Escherichia coli RNA polymerase
Author(s) -
S P Ridley,
Max P. Oeschger
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.152.2.736-746.1982
Subject(s) - biology , escherichia coli , mutagenesis , microbiology and biotechnology , gene , mutation , rna polymerase , genetics , polymerase , protein subunit , beta (programming language) , allele , computer science , programming language
An Escherichia coli strain carrying an amber mutation (UAG) in rpoC, the gene encoding the beta prime subunit of RNA polymerase, was isolated after mutagenesis with nitrosoguanidine. The mutation was moved into an unmutagenized strain carrying the supD43,74 allele, which encodes a temperature-sensitive su1 amber suppressor, and sue alleles, which enhance the efficiency of the suppressor. In this background, beta prime is not synthesized at high temperature. Suppression of the mutation by the non-temperature-sensitive amber suppressor su1+ yields a protein which is functional at all temperatures examined (30, 37, and 42 degrees C).