Open AccessReduction of exogenous cytochrome c by Neurospora crassa conidia: effects of superoxide dismutase and blue light
Author(s) -
Umeo Takahama,
M. Shimizu-Takahama,
Takeshi Egashira
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.152.1.151-156.1982
Subject(s) - neurospora crassa , superoxide dismutase , cytochrome c , cytochrome , biology , biochemistry , cytochrome c peroxidase , antimycin a , flavin group , enzyme , electron transport chain , mitochondrion , gene , mutant
The reduction of externally added cytochrome c by Neurospora crassa conidia was observed. The reduction was stimulated by antimycin A and suppressed partially by superoxide dismutase. When conidia were treated with diethyldithiocarbamate, which inactivated endogenous superoxide dismutase, the cytochrome c reduction was stimulated. Blue light also stimulated the cytochrome c reduction. Azide, which inhibits photochemical reactions mediated by flavins, suppressed the blue light effect. Superoxide dismutase partially suppressed the cytochrome c reduction in the light. The results suggest that O2(-) participates in the cytochrome c reduction by conidia and the flavins or flavoproteins are candidates for the receptor pigment of blue light to stimulate the cytochrome c reduction. It was also suggested that the redox component(s), which could directly transfer its reducing equivalents to exogenous cytochrome c, was present at the surface of conidia.