Open AccessPromoter mapping and selection of operator mutants by using insertion of bacteriophage Mu in the argECBH divergent operon of Escherichia coli K-12
Author(s) -
G Beny,
A Boyen,
Daniël Charlier,
Willy Lissens,
A. Feller,
Nicolas Glansdorff
Publication year - 1982
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.151.1.62-67.1982
Subject(s) - operon , biology , bacteriophage , mutant , operator (biology) , lac operon , genetics , escherichia coli , gene , transcription (linguistics) , promoter , mutation , microbiology and biotechnology , transcription factor , repressor , gene expression , linguistics , philosophy
The analysis of a large number of Arg mutants obtained by inserting phage Mu in the argECBH cluster of genes confirmed the "facing" arrangement proposed earlier for the promoters of argE (argEp) and argCBH (argCBHp) and clarified remaining ambiguities regarding the localization of argEp. Casadaban and Cohen's Mu d lac phages (M. Casadaban and S. N. Cohen, Proc. Natl. Acad. Sci. U.S.A. 76:4530-4533, 1979) were used to construct strains where either an intact or a truncated lacZ gene was fused to argC or argB. Several operator-constitutive mutations could be selected for in such strains; the mutations affected both arms of the cluster, thereby defining one common operator region for both directions of transcription.
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